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產(chǎn)品名稱(chēng):GSTSelector
產(chǎn)品型號(hào):
產(chǎn)品廠(chǎng)商:Nano-Tag
產(chǎn)品文檔:
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簡(jiǎn)單介紹:
GSTSelector
詳情介紹:
Product details
Product details
Sample Type
Cell Extracts
Specificity
The
Antibody
with Clone G19 recognizes Schistosoma japonicum Glutathione S-transferase (GST)
Characteristics
GST Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4?% cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. GST Selector thus features high affinity and superior capacity for GST fusion proteins while showing negligible non-specific background. GST Selector is compatible not only with physiological buffers but also with high stringency buffers. GST Selector thus provides great freedom to adjust the binding and washing conditions to the experimental needs.
Components
4 % cross-linked agarose (bead size 50-150 μm) with covalently immobilized single-domain antibody
Material not included
wash buffers, columns, tubes
ProductDetails: Bead Ligand
Antibody
ProductDetails: Bead Matrix
Agarose beads
ProductDetails: Bead Size
90 μm
Target details
Target details
Alternative Name
Glutathione S-transferase (GST)
Background
Glutathione S-transferase (GST) from Schistosoma japonicum is a 26 kDa enzyme, often found as a fusion partner in expression systems. GST folds rapidly and boosts the expression and the folding of the fusion partner. It is also used as purification tag wi
Application Details
Application Details
Application Notes
200?μL slurry for up to 10 reactions
Comment
Capacity: > 4 μg GST per μl of packed beads (i.e. 2 μl of slurry)
Restrictions
For Research Use only
Handling
Handling
Buffer
50?% slurry in 20?% ethanol / PBS 0.09?% sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C